A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility

被引：49

作者：

Ichikawa, N

Kishimoto, N

Inagaki, A

Nakamura, A

Koshino, Y

Yokozeki, Y

Oka, M

Samoto, S

Akagi, H

Higo, K

Shinjyo, C

Fujimura, T

Shimada, H

机构：

[1] MITSUI TOATSU CHEM CO LTD,INST LIFE SCI,MOBARA 297,JAPAN

[2] NATL INST AGROBIOL RESOURCES,TSUKUBA,IBARAKI 305,JAPAN

[3] UNIV RYUKYUS,COLL AGR,OKINAWA 90301,JAPAN

来源：

MOLECULAR BREEDING | 1997年 / 3卷 / 03期

关键词：

cytoplasmic male sterility; genetic diagnosis; hybrid rice seeds; molecular marker-assisted breeding; restore gene; RFLP linkage map;

D O I：

10.1023/A:1009601007175

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Cytoplasmic male sterility (CMS) is widely known in higher plants, the mechanism of which is believed to involve incompatibility between nuclei and cytoplasms. In rice lines with the CMS trait, fertility is restored by the aid of a nuclear-encoded gene, Rf-1, whose locus has been determined in chromosome 10. We found a particular PCR-amplified fragment, designated fL601, that specifically amplified using the DNAs from Rf-1 lines tested as templates. RFLP mapping of the fL601 locus revealed that there are two loci for the fL601, and that both are tightly linked to the Rf-1 locus. Progeny analysis also showed high frequency of their co-segregation. Southern analysis of the genomic DNA demonstrated that the Rf-1 lines shared a unique sequence in the fL601 region. These results enabled us to construct a system for specific detection of the corresponding regions. Utilizing this detection system, we established a simple PCR-mediated selection method for the Rf-1 lines, which may facilitate the breeding for hybrid rice.

引用

页码：195 / 202

页数：8

共 37 条

[11]

KATSUO K., 1958, JAPANESE JOUR BREEDING, V8, P1

[12] INITIATION AND PROCESSING OF ATP6, T-URF13 AND ORF221 TRANSCRIPTS FROM MITOCHONDRIA OF T-CYTOPLASM MAIZE [J].

KENNELL, JC ;

PRING, DR .

MOLECULAR & GENERAL GENETICS, 1989, 216 (01) :16-24

[13]

KHUSH GS, 1990, RICE GENET NEWSL, V7, P12

[14]

KINOSHITA T, 1987, RICE GENET NEWSLETT, V4, P3

[15] THE CODING SEQUENCE FOR RICE SEED CATALASE DETECTS A LOCUS DIFFERENT FROM THAT DETERMINED BY ISOZYME ANALYSIS [J].

KISHIMOTO, N ;

HIGO, K ;

HIGO, H ;

SAITO, A .

THEORETICAL AND APPLIED GENETICS, 1994, 87 (06) :625-626

[16] A 300 KILOBASE INTERVAL GENETIC-MAP OF RICE INCLUDING 883 EXPRESSED SEQUENCES [J].

KURATA, N ;

NAGAMURA, Y ;

YAMAMOTO, K ;

HARUSHIMA, Y ;

SUE, N ;

WU, J ;

ANTONIO, BA ;

SHOMURA, A ;

SHIMIZU, T ;

LIN, SY ;

INOUE, T ;

FUKUDA, A ;

SHIMANO, T ;

KUBOKI, Y ;

TOYAMA, T ;

MIYAMOTO, Y ;

KIRIHARA, T ;

HAYASAKA, K ;

MIYAO, A ;

MONNA, L ;

ZHONG, HS ;

TAMURA, Y ;

WANG, ZX ;

MOMMA, T ;

UMEHARA, Y ;

YANO, M ;

SASAKI, T ;

MINOBE, Y .

NATURE GENETICS, 1994, 8 (04) :365-372

[17] CYTOPLASMIC MALE-STERILITY IN MAIZE [J].

LAUGHNAN, JR ;

GABAYLAUGHNAN, S .

ANNUAL REVIEW OF GENETICS, 1983, 17 :27-48

[18] MOLECULAR-BIOLOGY OF PLANT-MITOCHONDRIA [J].

LEVINGS, CS ;

BROWN, GG .

CELL, 1989, 56 (02) :171-179

[19]

LI Z, 1986, HYBRID RICE, P85

[20] RAPID IDENTIFICATION OF MARKERS LINKED TO A PSEUDOMONAS RESISTANCE GENE IN TOMATO BY USING RANDOM PRIMERS AND NEAR-ISOGENIC LINES [J].

MARTIN, GB ;

WILLIAMS, JGK ;

TANKSLEY, SD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2336-2340

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