Passive conductivity detection for capillary electrophoresis

被引：27

作者：

Bai, XX ^{[1
]}

Wu, ZY ^{[1
]}

Josserand, J ^{[1
]}

Jensen, H ^{[1
]}

Schafer, H ^{[1
]}

Girault, HH ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chim Mol & Biol, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 11期

关键词：

D O I：

10.1021/ac035462k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A passive electrochemical detection principle that can be applied to capillary electrophoresis is presented. The separation electrical field is used to generate a potential difference between two electrodes located along the channel. For constant-current electrophoresis, the generated signal is proportional to the resistance of the solution passing between the two electrodes. Contrary to conductivity detectors that are ac driven and need to be decoupled from the separation field, the passive detection directly takes advantage of the separation field. The signal is simply measured by a high-impedance voltmeter. The detection concept has been validated by numerical simulations showing how the magnitude of the signal is related to the ratio between the electrode distance and the length of the sample plug. As a proof of the principle, this detection concept has been demonstrated by the electrophoretic separation of three alkali ions on a polymer microchip. Based on preliminary results, a detection limit of 20 muM and a dynamic range of up to 3 orders of magnitude have been achieved.

引用

页码：3126 / 3131

页数：6

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