Imaging Transcription in Living Cells

被引：90

作者：

Darzacq, Xavier ^{[1
,2
]}

Yao, Jie ^{[1
,3
]}

Larson, Daniel R. ^{[1
,4
]}

Causse, Sebastien Z. ^{[1
,2
]}

Bosanac, Lana ^{[1
,2
]}

de Turris, Valeria ^{[1
,4
]}

Ruda, Vera M. ^{[1
,2
]}

Lionnet, Timothee ^{[1
,4
]}

Zenklusen, Daniel ^{[1
,4
]}

Guglielmi, Benjamin ^{[1
,3
]}

Tjian, Robert ^{[1
,3
]}

Singer, Robert H. ^{[1
,4
]}

机构：

[1] Howard Hughes Med Inst, Janelia Farm Res Consortium Imaging Transcript, Ashburn, VA 20147 USA

[2] Ecole Normale Super, CNRS, UMR 8541, F-75230 Paris 5, France

[3] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[4] Yeshiva Univ, Albert Einstein Coll Med, Dept Anat & Struct Biol, Gruss Lipper Biophoton Ctr, Bronx, NY 10461 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS | 2009年 / 38卷

关键词：

modeling; RNA synthesis in live cells; polymerase kinetics; RNA-POLYMERASE-II; LIGAND-SPECIFIC DYNAMICS; MESSENGER-RNA; GLUCOCORTICOID-RECEPTOR; GENE-EXPRESSION; REAL-TIME; FLUORESCENCE RECOVERY; NUCLEAR PERIPHERY; P-TEFB; PHOTOBLEACHING FRAP;

D O I：

10.1146/annurev.biophys.050708.133728

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription the kinetics of, polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.

引用

页码：173 / 196

页数：24

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