Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes

被引：15

作者：

Rindi, L ^{[1
]}

Lari, N ^{[1
]}

Bonanni, D ^{[1
]}

Garzelli, C ^{[1
]}

机构：

[1] Univ Pisa, Dipartimento Patol Sperimentale Biotecnol Med Inf, I-56127 Pisa, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 59卷 / 02期

关键词：

Mycobacterium tuberculosis; PCR; genes;

D O I：

10.1016/j.mimet.2004.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A duplex real-time PCR assay was developed for the assignment of Mycobacterium tuberculosis isolates to the three genotypic groups based on the katG463/gyrA95 polymorphism. The assay was as sensitive and specific as nucleotide sequencing and proved also able to detect unambiguously the isolate genotype in clinical specimens. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：283 / 287

页数：5

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