Evidence that heteronuclear proteins interact with the XIST RNA in vitro

被引:23
作者
Brown, CJ
Baldry, SEL
机构
[1] Department of Medical Genetics, University of British Columbia, Vancouver
关键词
D O I
10.1007/BF02369896
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The process of X chromosome inactivation results in the transcriptional silencing of one of the two X chromosomes in mammalian females. A large heterogeneous nuclear RNA that is expressed exclusively from the inactive X chromosome (XIST-X Inactive Specific Transcripts) has been implicated in the inactivation process. The XIST RNA colocalizes with the inactive X chromosome and therefore proteins that interact with the XIST RNA may be involved in the inactivation of the X chromosome. In order to identify such proteins we have used an in vitro UV light cross-linking technique to detect nuclear proteins associating with sections of the XIST RNA. The strongest interaction detected by this technique was between a pair of approximately 40 kDa proteins and a 5' region of the XIST RNA which contains a series of well-conserved tandem repeats, Immunoprecipitation suggested that these proteins may be the heteronuclear proteins hnRNPC1/C2.
引用
收藏
页码:403 / 417
页数:15
相关论文
共 54 条
[1]   RETROVIRAL INSERTIONS IN THE MURINE HIS-1 LOCUS ACTIVATE THE EXPRESSION OF A NOVEL RNA THAT LACKS AN EXTENSIVE OPEN READING FRAME [J].
ASKEW, DS ;
LI, J ;
IHLE, JN .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) :1743-1751
[2]  
BARR ML, 1962, ACTA CYTOL, V6, P34
[3]  
BELGRADER P, 1991, J CELL SCI, V98, P281
[4]   A 348-base-pair region in the latency-associated transcript facilitates herpes simplex virus type 1 reactivation [J].
Bloom, DC ;
Hill, JM ;
DeviRao, G ;
Wagner, EK ;
Feldman, LT ;
Stevens, JG .
JOURNAL OF VIROLOGY, 1996, 70 (04) :2449-2459
[5]   THE PRODUCT OF THE H19 GENE MAY FUNCTION AS AN RNA [J].
BRANNAN, CI ;
DEES, EC ;
INGRAM, RS ;
TILGHMAN, SM .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (01) :28-36
[6]   THE PRODUCT OF THE MOUSE XIST GENE IS A 15 KB INACTIVE X-SPECIFIC TRANSCRIPT CONTAINING NO CONSERVED ORF AND LOCATED IN THE NUCLEUS [J].
BROCKDORFF, N ;
ASHWORTH, A ;
KAY, GF ;
MCCABE, VM ;
NORRIS, DP ;
COOPER, PJ ;
SWIFT, S ;
RASTAN, S .
CELL, 1992, 71 (03) :515-526
[7]   LOCALIZATION OF THE X-INACTIVATION CENTER ON THE HUMAN X-CHROMOSOME IN XQ13 [J].
BROWN, CJ ;
LAFRENIERE, RG ;
POWERS, VE ;
SEBASTIO, G ;
BALLABIO, A ;
PETTIGREW, AL ;
LEDBETTER, DH ;
LEVY, E ;
CRAIG, IW ;
WILLARD, HF .
NATURE, 1991, 349 (6304) :82-84
[8]   THE DXS423E GENE IN XP11.21 ESCAPES X-CHROMOSOME INACTIVATION [J].
BROWN, CJ ;
MILLER, AP ;
CARREL, L ;
RUPERT, JL ;
DAVIES, KE ;
WILLARD, HF .
HUMAN MOLECULAR GENETICS, 1995, 4 (02) :251-255
[9]   THE HUMAN XIST GENE - ANALYSIS OF A 17 KB INACTIVE X-SPECIFIC RNA THAT CONTAINS CONSERVED REPEATS AND IS HIGHLY LOCALIZED WITHIN THE NUCLEUS [J].
BROWN, CJ ;
HENDRICH, BD ;
RUPERT, JL ;
LAFRENIERE, RG ;
XING, Y ;
LAWRENCE, J ;
WILLARD, HF .
CELL, 1992, 71 (03) :527-542
[10]   ISOLATION OF THE HETEROGENEOUS NUCLEAR-RNA RIBONUCLEOPROTEIN COMPLEX (HNRNP) - A UNIQUE SUPRAMOLECULAR ASSEMBLY [J].
CHOI, YD ;
DREYFUSS, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7471-7475