共 49 条
Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells
被引:164
作者:
Kalaszczynska, Ilona
[1
,4
]
Geng, Yan
[1
,4
]
Iino, Tadafumi
[2
,4
]
Mizuno, Shin-ichi
[2
,4
]
Choi, Yoon
[1
,4
]
Kondratiuk, Ilona
[1
,4
]
Silver, Daniel P.
[3
,5
]
Wolgemuth, Debra J.
[6
,7
]
Akashi, Koichi
[2
,4
]
Sicinski, Piotr
[1
,4
]
机构:
[1] Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA
[2] Dana Farber Canc Inst, Dept Canc Immunol & AIDS, Boston, MA 02115 USA
[3] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA
[5] Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA
[6] Columbia Univ, Ctr Med, Dept Genet & Dev, New York, NY 10032 USA
[7] Columbia Univ, Ctr Med, Dept Obstet & Gynecol, New York, NY 10032 USA
来源:
基金:
美国国家卫生研究院;
关键词:
DEPENDENT KINASES;
C-MYC;
EXPRESSION;
PROGRESSION;
GENE;
MICE;
MOUSE;
PROLIFERATION;
ACCUMULATION;
PROGENITOR;
D O I:
10.1016/j.cell.2009.04.062
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.
引用
收藏
页码:352 / 365
页数:14
相关论文