Mutations in Helicobacter pylori porD and oorD genes may contribute to furazolidone resistance

被引：20

作者：

Su, Zhaoliang ^{[1
]}

Xu, Huaxi ^{[1
]}

Zhang, Chiyu ^{[1
]}

Shao, Shihe ^{[1
]}

Li, Liangju ^{[1
]}

Wang, Hua ^{[1
]}

Wang, Huifang ^{[1
]}

Qiu, Gufeng ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Med Technol, Inst Clin Lab Sci, Dept Microbiol & Immunol, Zhenjiang City 212001, Jiangsu Prov, Peoples R China

来源：

CROATIAN MEDICAL JOURNAL | 2006年 / 47卷 / 03期

关键词：

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Aim To determine the rate of furazolidone resistance of Helicobacter pylori (H. pylori) isolated from gastric biopsy specimens and to explore the relationship between genetic mutations in porD and oorD genes of H. pylori and its resistance to the antibiotic. Methods Gastric biopsy was performed in 83 adult patients aged 31-77 years with gastric complaints. H. pylori was isolated from biopsy specimens of 46 patients. E-test and 2-fold agar dilution method were used to determine the rate of H.pylori resistance to fiarazolidone, The genes porD and oorD from susceptible and resistant isolates were amplified by polymerase chain reaction (PCR), and their PCR products were sequenced. Results Resistance to furazolidone was found in 8.796 of H. pylori isolates and 6 mutations were detected in porD and oorD genes of the. resistant isolates. Three mutations - G353A, A356G, and C357T - occurred in porD and the other mutations - A041G, A122G, C349A(G) - occurred in oorD genes. Conclusions Changes in 6 amino acids may be associated with the resistance of H. pylori to furazolidone.

引用

页码：410 / 415

页数：6

共 23 条

[1]

Bactericidal and morphological effects of NE-2001, a novel synthetic agent directed against Helicobacter pylori [J].