A New Regeneration System for Oxidized Nicotinamide Cofactors

被引：52

作者：

Aksu, Seda ^{[1
]}

Arends, Isabel W. C. E. ^{[1
]}

Hollmann, Frank ^{[1
]}

机构：

[1] Delft Univ Technol, Dept Biotechnol, NL-2628 BL Delft, Netherlands

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2009年 / 351卷 / 09期

关键词：

aerobic oxidation; cofactors; enzyme catalysis; laccase mediator system; oxidoreductases; CONTINUOUS ENZYMATIC REGENERATION; TRANSITION-METAL-COMPLEXES; NADH OXIDASE; NONENZYMATIC REGENERATION; ALCOHOL-DEHYDROGENASE; LACTOBACILLUS-BREVIS; OXIDATION; EFFICIENT; NAD(+); ACID;

D O I：

10.1002/adsc.200900033

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel regeneration system for oxidized nicotinamide cofactors (NAD(+) and NADP(+)) is presented. By combining 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS)-catalyzed oxidation of NAD(P)H with laccase-catalyzed utilization of molecular oxygen as terminal oxidant, a simple chemo-enzymatic NAD(P)(+) regeneration method is achieved. Thus, the advantages of both worlds, chemical oxidation of reduced nicotinamide cofactors and laccase-catalyzed utilization of oxygen from air are combined in a simple and generally applicable new approach for biooxidation catalysis. This new application of the well-known laccase-mediator system (LMS) is successfully used to promote alcohol dehydrogenase-catalyzed oxidation reactions of primary and secondary alcohols. Already under non-optimized conditions, high turnover numbers of > 300 and > 16000 were obtained for the nicotinamide cofactor and ABTS, respectively. In this communication, we present the proof-of-principle and initial characterization of the proposed new regeneration system.

引用

页码：1211 / 1216

页数：6

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