Manipulation of an Innate Escape Response in Drosophila: Photoexcitation of acj6 Neurons Induces the Escape Response

被引：18

作者：

Zimmermann, Gregor ^{[1
]}

Wang, Li-ping ^{[2
]}

Vaughan, Alexander G. ^{[3
]}

Manoli, Devanand S. ^{[3
]}

Zhang, Feng ^{[2
]}

Deisseroth, Karl ^{[2
]}

Baker, Bruce S. ^{[3
]}

Scott, Matthew P. ^{[1
]}

机构：

[1] Stanford Univ, Dept Dev Biol, Dept Genet, Dept Bioengn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn & Psychiat, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

PLOS ONE | 2009年 / 4卷 / 04期

关键词：

D O I：

10.1371/journal.pone.0005100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: The genetic analysis of behavior in Drosophila melanogaster has linked genes controlling neuronal connectivity and physiology to specific neuronal circuits underlying a variety of innate behaviors. We investigated the circuitry underlying the adult startle response, using photoexcitation of neurons that produce the abnormal chemosensory jump 6 (acj6) transcription factor. This transcription factor has previously been shown to play a role in neuronal pathfinding and neurotransmitter modality, but the role of acj6 neurons in the adult startle response was largely unknown. Principal Findings: We show that the activity of these neurons is necessary for a wild-type startle response and that excitation is sufficient to generate a synthetic escape response. Further, we show that this synthetic response is still sensitive to the dose of acj6 suggesting that that acj6 mutation alters neuronal activity as well as connectivity and neurotransmitter production. Results/Significance: These results extend the understanding of the role of acj6 and of the adult startle response in general. They also demonstrate the usefulness of activity-dependent characterization of neuronal circuits underlying innate behaviors in Drosophila, and the utility of integrating genetic analysis into modern circuit analysis techniques.

引用

页数：10