Expression of CD109 in human cancer

被引:72
作者
Hashimoto, M
Ichihara, M
Watanabe, T
Kawai, K
Koshikawa, K
Yuasa, N
Takahashi, T
Yatabe, Y
Murakumo, Y
Zhang, JM
Nimura, Y
Takahashi, M
机构
[1] Nagoya Univ, Grad Sch Med, Dept Pathol, Showa Ku, Nagoya, Aichi 4668550, Japan
[2] Nagoya Univ, Grad Sch Med, Dept Surg, Showa Ku, Nagoya, Aichi 4668550, Japan
[3] Nagoya Univ, Grad Sch Med, Ctr Neural Dis & Canc, Div Mol Pathol,Showa Ku, Nagoya, Aichi 4668550, Japan
[4] Aichi Canc Ctr, Res Inst, Div Mol Oncol, Chikusa Ku, Nagoya, Aichi 4648681, Japan
[5] Aichi Canc Ctr, Dept Pathol & Mol Diagnost, Chikusa Ku, Nagoya, Aichi 4648681, Japan
关键词
CD109; squamous cell carcinoma; lung carcinoma; esophageal carcinoma;
D O I
10.1038/sj.onc.1207418
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It was recently reported that the human CD109 gene encodes a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the alpha(2)-macroglobulin/C3, C4, C5 family of thioester-containing proteins. In this study, we found that the expression of mouse CD109 gene was upregulated in NIH3T3 cells expressing RET tyrosine kinase with a multiple endocrine neoplasia 2B mutation. Northern blot analysis showed a high level of expression of the CD109 gene only in the testis in normal human and mouse tissues. In addition, its expression was high in some human tumor cell lines, which included squamous cell carcinoma and glioblastoma cell lines, whereas it was undetectable in neuroblastoma and small-cell lung carcinoma cell lines. When CD109 expression was examined in 33 cases of human lung cell carcinomas by quantitative RT-PCR, a significant high expression of CD109 was detected in about half of squamous cell carcinomas examined, but not in adenocarcinoma, large-cell carcinoma and small-cell carcinoma. Similarly, upregulation of CD109 was observed in nine out of 17 esophageal squamous cell carcinomas. Thus, these results suggested that CD109 might be a useful molecular target for the development of new therapeutics for malignant tumors, such as squamous cell carcinoma.
引用
收藏
页码:3716 / 3720
页数:5
相关论文
共 13 条
[1]   CELLULAR EXPRESSION OF A GPI-LINKED T-CELL ACTIVATION PROTEIN [J].
HAREGEWOIN, A ;
SOLOMON, K ;
HOM, RC ;
SOMAN, G ;
BERGELSON, JM ;
BHAN, AK ;
FINBERG, RW .
CELLULAR IMMUNOLOGY, 1994, 156 (02) :357-370
[2]   The PROSITE database, its status in 1999 [J].
Hofmann, K ;
Bucher, P ;
Falquet, L ;
Bairoch, A .
NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :215-219
[3]   Cell surface antigen CD109 is a novel member of the α2 macroglobulin/C3, C4, C5 family of thioester-containing proteins [J].
Lin, M ;
Sutherland, R ;
Horsfall, W ;
Totty, N ;
Yeo, E ;
Nayar, R ;
Wu, XF ;
Schuh, AC .
BLOOD, 2002, 99 (05) :1683-1691
[4]   CD109 is expressed on a subpopulation of CD34+ cells enriched in hematopoietic stem and progenitor cells [J].
Murray, LJ ;
Bruno, E ;
Uchida, N ;
Hoffman, R ;
Nayar, R ;
Yeo, EL ;
Schuh, AC ;
Sutherland, DR .
EXPERIMENTAL HEMATOLOGY, 1999, 27 (08) :1282-1294
[5]  
Rappold I, 1997, BLOOD, V90, P111
[6]  
SMITH JW, 1995, BLOOD, V86, P2807
[7]   Determination of the non-ionic detergent insolubility and phosphoprotein associations of glycosylphosphatidylinositol-anchored proteins expressed on T cells [J].
Solomon, KR ;
Mallory, MA ;
Finberg, RW .
BIOCHEMICAL JOURNAL, 1998, 334 :325-333
[8]  
SUTHERLAND DR, 1991, BLOOD, V77, P84
[9]  
SUTHERLAND DR, 1998, LEUKOCYTE TYPING, V6, P714
[10]  
SUTHERLAND DR, 1995, LEUKOCYTE TYPING, V5, P1767