Acetic acid activates PKD1L3-PKD2L1 channel-A candidate sour taste receptor

被引:38
作者
Ishii, Sho [1 ,2 ]
Misaka, Takumi [1 ]
Kishi, Mikiya [2 ]
Kaga, Takayuki [2 ]
Ishimaru, Yoshiro [1 ]
Abe, Keiko [1 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
[2] Mizkan Grp Co, Cent Res Inst, Aichi 4758585, Japan
关键词
Acetic acid; Taste; Sour; PKD1L3; PKD2L1; RATS; INTENSITY;
D O I
10.1016/j.bbrc.2009.05.069
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The polycystic kidney disease (PKD) 1L3-PKD2L1 channel is a candidate sour taste receptor expressed in mammalian taste receptor cells. Various acids are reported to activate PKD channels after the removal of the acid stimuli, but little information is available on the activation of these channels by acetic acid. It was difficult to analyze the PKD channel activation by acetic acid using Ca2+ imaging experiments because this acid induces a transient and nonspecific response in cultured cells. Here, we developed a novel method to evaluate PKD channel activation by acetic acid. Nonspecific responses were observed only over a short period after the application of acetic acid, In contrast, PKD channel activation evoked by acetic acid as well as citric acid was detected even at a later time point. This method revealed that PKD1L3-PKD2L1 channel activation by acetic acid was pH-dependent and occurred when the ambient pH was <3.1. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:346 / 350
页数:5
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