Physiological stresses increase mouse short interspersed element (SINE) RNA expression in vivo

被引:117
作者
Li, TH
Spearow, J
Rubin, CM
Schmid, CW [1 ]
机构
[1] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA
[2] Univ Calif Davis, Sect Neurobiol Physiol & Behav, Davis, CA 95616 USA
[3] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
关键词
heat shock; SINE; stress; transcription;
D O I
10.1016/S0378-1119(99)00384-4
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The possible functionality of short interspersed elements (SINEs) is investigated by assaying the effects of physiological stress on their RNA polymerase-III-directed transcriptional expression in vivo. B2 RNA is expressed at moderately high levels in all mouse tissues investigated, namely liver, spleen, kidney and testis. III RNA is expressed in testis but is nearly undetectable in the other tissues. Following hyperthermic shock, the amounts of B1 and B2 SINE RNAs transiently increase in all tissues by as much as 40-fold in certain cases. The kinetics of these increases resemble those of heat shock protein mRNAs. An acute dose of ethanol also transiently increases the abundance of B1 and B2 RNA in liver, showing that other physiological stresses increase SINE RNA expression. The constitutive expression of B2 RNA in all tissues and tissue-specific differences in expression of B1 RNA imply that these transcripts serve a normal physiological function(s). Moreover, increased SINE RNA expression is a vital response to stress and by the criterion of their inducibility, mammalian SINEs behave like regulated cell stress genes. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:367 / 372
页数:6
相关论文
共 29 条
[1]   Localization of heat shock proteins in mouse male germ cells: An immunoelectron microscopical study [J].
Biggiogera, M ;
Tanguay, RM ;
Marin, R ;
Wu, Y ;
Martin, TE ;
Fakan, S .
EXPERIMENTAL CELL RESEARCH, 1996, 229 (01) :77-85
[2]   Ethanol teratogenesis in the C57BL/6J, DBA/2J, and A/J inbred mouse strains [J].
Boehm, SL ;
Lundahl, KR ;
Caldwell, J ;
Gilliam, DM .
ALCOHOL, 1997, 14 (04) :389-395
[3]   Stress proteins and sh-groups in oxidant-induced cell damage after acute ethanol administration in rat [J].
Calabrese, V ;
Renis, M ;
Calderone, A ;
Russo, A ;
Barcellona, ML ;
Rizza, V .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 20 (03) :391-397
[4]   INDUCTION OF SPECIFIC TRANSCRIPTION BY RNA POLYMERASE-III IN TRANSFORMED-CELLS [J].
CAREY, MF ;
SINGH, K ;
BOTCHAN, M ;
COZZARELLI, NR .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (09) :3068-3076
[5]   ENHANCED B2 TRANSCRIPTION IN SIMIAN VIRUS-40-TRANSFORMED CELLS IS MEDIATED THROUGH THE FORMATION OF RNA POLYMERASE-III TRANSCRIPTION COMPLEXES ON PREVIOUSLY INACTIVE GENES [J].
CAREY, MF ;
SINGH, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7059-7063
[6]   THE EVOLUTIONARY DYNAMICS OF REPETITIVE DNA IN EUKARYOTES [J].
CHARLESWORTH, B ;
SNIEGOWSKI, P ;
STEPHAN, W .
NATURE, 1994, 371 (6494) :215-220
[7]   Potential Alu function: Regulation of the activity of double-stranded RNA-activated kinase PKR [J].
Chu, WM ;
Ballard, R ;
Carpick, BW ;
Williams, BRG ;
Schmid, CW .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (01) :58-68
[8]   SELFISH GENES, THE PHENOTYPE PARADIGM AND GENOME EVOLUTION [J].
DOOLITTLE, WF ;
SAPIENZA, C .
NATURE, 1980, 284 (5757) :601-603
[9]   INDUCTION OF B2 RNA POLYMERASE-III TRANSCRIPTION BY HEAT-SHOCK - ENRICHMENT FOR HEAT-SHOCK INDUCED SEQUENCES IN RODENT CELLS BY HYBRIDIZATION SUBTRACTION [J].
FORNACE, AJ ;
MITCHELL, JB .
NUCLEIC ACIDS RESEARCH, 1986, 14 (14) :5793-5811
[10]   HYPERTHERMIA PROTECTS MICE AGAINST THE LETHAL EFFECTS OF ENDOTOXIN [J].
HOTCHKISS, R ;
NUNNALLY, I ;
LINDQUIST, S ;
TAULIEN, J ;
PERDRIZET, G ;
KARL, I .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (06) :R1447-R1457