Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis

被引:117
作者
Antonio Mata, Juan
Bejar, Victoria
Llamas, Inmaculada
Arias, Soledad
Bressollier, Philippe
Tallon, Richard
Urdaci, Maria C.
Quesada, Emilia
机构
[1] Univ Granada, Microbial Exopolysaccharide Res Grp, Dept Microbiol, Fac Pharm, E-18071 Granada, Spain
[2] IUT, Dept Genie Biol, F-87065 Limoges, France
[3] ENITA Bordeaux, Lab Microbiol & Biochim Appl, F-33175 Gradignan, France
关键词
exopolysaccharides; Halomonas ventosae; Holomonas anticariensis; halophilic bacteria;
D O I
10.1016/j.resmic.2006.06.004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains A112(T) and A116 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg of EPS per gram of dry cell weight) respectively. The molecular masses of the polymers were about 50 kDa and their main components were glucose, mannose and galactose. They had high protein fractions and showed emulsifying activity on several hydrophobic substrates. Under optimum environmental and nutritional conditions, H. anticariensis strains FP35(T) and FP36 excreted about 29.65 and 49.95 mg of EPS per 100 ml of culture medium (43.6 and 50.95 mg of EPS per gram of dry cell weight) respectively. The molecular masses of the polymers were about 20 and 46 kDa respectively and were composed mainly of glucose, mannose and galacturonic acid. All EPSs produced solutions of low viscosity and pseudoplastic behaviour. They also had a high capacity for binding cations and incorporated considerable quantities of sulphates, which is highly unusual in bacterial polysaccharides. All strains assayed formed biofilms both in polystyrene wells and borosilicate test tubes. (c) 2006 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:827 / 835
页数:9
相关论文
共 62 条
[1]  
ALBAN S, 1997, CARBOHYDRATES DRUG D, P209
[2]  
ALLISON DG, 1987, J GEN MICROBIOL, V133, P1319
[3]  
ANTON J, 1988, APPL ENVIRON MICROB, V54, P2381
[4]  
ARFORS KE, 1993, J LAB CLIN MED, V121, P201
[5]   Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology [J].
Arias, S ;
del Moral, A ;
Ferrer, MR ;
Tallon, R ;
Quesada, E ;
Béjar, V .
EXTREMOPHILES, 2003, 7 (04) :319-326
[6]   EMULSIFYING PROPERTY OF A VISCOUS EXOPOLYSACCHARIDE FROM SPHINGOMONAS-PAUCIMOBILIS [J].
ASHTAPUTRE, AA ;
SHAH, AK .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1995, 11 (02) :219-222
[7]   Characterization of exopolysaccharides produced by 19 halophilic strains of the species Halomonas eurihalina [J].
Béjar, V ;
Llamas, I ;
Calvo, C ;
Quesada, E .
JOURNAL OF BIOTECHNOLOGY, 1998, 61 (02) :135-141
[8]  
BERGMEYER HU, 1965, METHOD ENZYMAT AN, P123
[9]  
Boisson-Vidal C., 1995, Drugs of the Future, V20, P1237
[10]   Halomonas maura sp nov., a novel moderately halophilic, exopolysaccharide-producing bacterium [J].
Bouchotroch, S ;
Quesada, E ;
del Moral, A ;
Llamas, I ;
Béjar, V .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1625-1632