Use of the omp50 gene for identification of Campylobacter species by PCR

被引：20

作者：

Dedieu, L

Pagès, JM

Bolla, JM

机构：

[1] Univ Mediterranee, Fac Med, EA 2197, F-13385 Marseille 5, France

[2] Univ Mediterranee, Fac Med, IFR 48, F-13385 Marseille 5, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 05期

关键词：

D O I：

10.1128/JCM.42.5.2301-2305.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

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页码：2301 / 2305

页数：5

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