BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling

被引:1134
作者
Li, J
Wen, JQ
Lease, KA
Doke, JT
Tax, FE
Walker, JC [1 ]
机构
[1] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA
[2] Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/S0092-8674(02)00812-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brassinosteroids regulate plant growth and development through a protein complex that includes the leucine-rich repeat receptor-like protein kinase (LRR-RLK) brassinosteroid-insensitive 1 (BRI1). Activation tagging was used to identify a dominant genetic suppressor of bri1, bak1-1D (bri1-associated receptor kinase 1-1Dominant), which encodes an LRR-RLK, distinct from BRI1. Overexpression of BAK1 results in elongated organ phenotypes, while a null allele of BAK1 displays a semidwarfed phenotype and has reduced sensitivity to brassinosteroids (BRs). BAK1 is a serine/threonine protein kinase, and BRI1 and BAK1 interact in vitro and in vivo. Expression of a dominant-negative mutant allele of BAK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles. These results indicate BAK1 is a component of BR signaling.
引用
收藏
页码:213 / 222
页数:10
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