A microRNA detection system based on padlock probes and rolling circle amplification

被引：187

作者：

Jonstrup, Soren Peter

Koch, Jorn

Kjems, Jorgen

机构：

[1] Aarhus Univ, Dept Mol Biol, DK-8000 Aarhus C, Denmark

[2] Aarhus Sygehus, Dept Pathol, DK-8000 Aarhus C, Denmark

来源：

RNA | 2006年 / 12卷 / 09期

关键词：

microRNA; padlock probes; rolling circle;

D O I：

10.1261/rna.110706

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The differential expression and the regulatory roles of microRNAs ( miRNAs) are being studied intensively these years. Their minute size of only 19-24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA.

引用

收藏

页码：1747 / 1752

页数：6

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