Reassessing the role of phospholipase D in the Arabidopsis wounding response

被引:66
作者
Bargmann, Bastiaan O. R. [2 ]
Laxalt, Ana M. [2 ]
Ter Riet, Bas [2 ]
Testerink, Christa [2 ]
Merquiol, Emmanuelle [3 ]
Mosblech, Alina [4 ]
Leon-Reyes, Antonio [5 ]
Pieterse, Corne M. J. [5 ]
Haring, Michel A. [2 ]
Heilmann, Ingo [4 ]
Bartels, Dorothea [3 ]
Munnik, Teun [1 ]
机构
[1] Univ Bonn, D-53115 Bonn, Germany
[2] Univ Amsterdam, Swammerdam Inst Life Sci, Dept Plant Physiol, NL-1098 SM Amsterdam, Netherlands
[3] Vrije Univ Amsterdam, Dept Ecol & Physiol Plants, NL-1081 HV Amsterdam, Netherlands
[4] Univ Gottingen, Dept Plant Biochem, Albrecht von Haller Inst Plant Sci, D-37077 Gottingen, Germany
[5] Univ Utrecht, NL-3584 CH Utrecht, Netherlands
关键词
jasmonic acid; phosphatidic acid; PLD; G-PROTEIN ACTIVATION; PHOSPHATIDIC-ACID; JASMONIC ACID; DIACYLGLYCEROL PYROPHOSPHATE; SIGNALING PATHWAYS; NOD FACTOR; TOMATO; CHLAMYDOMONAS; ACCUMULATION; MECHANISM;
D O I
10.1111/j.1365-3040.2009.01962.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis (Arabidopsis thaliana) AtPLD alpha 1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using P-32(i)-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD-silenced tomato cell-suspension cultures. pld alpha 1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a pld alpha 1/delta double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly (Pieris rapae) grew equally well on wild-type and the pld knock-out mutants.
引用
收藏
页码:837 / 850
页数:14
相关论文
共 58 条
[1]   A defect in glyoxysomal fatty acid β-oxidation reduces jasmonic acid accumulation in Arabidopsis [J].
Afitlhile, MM ;
Fukushige, H ;
Nishimura, M ;
Hildebrand, DF .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2005, 43 (06) :603-609
[2]   Jasmonic acid is a key regulator of spider mite-induced volatile terpenoid and methyl salicylate emission in tomato [J].
Ament, K ;
Kant, MR ;
Sabelis, MW ;
Haring, MA ;
Schuurink, RC .
PLANT PHYSIOLOGY, 2004, 135 (04) :2025-2037
[3]   Jasmonate-induced responses are costly but benefit plants under attack in native populations [J].
Baldwin, IT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (14) :8113-8118
[4]   The role of phospholipase D in plant stress responses [J].
Bargmann, Bastiaan O. ;
Munnik, Teun .
CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (05) :515-522
[5]   LePLDβ1 activation and relocalization in suspension-cultured tomato cells treated with xylanase [J].
Bargmann, BOR ;
Laxalt, AM ;
ter Riet, B ;
Schouten, E ;
van Leeuwen, W ;
Dekker, HL ;
de Koster, CG ;
Haring, MA ;
Munnik, T .
PLANT JOURNAL, 2006, 45 (03) :358-368
[6]   CHARACTERIZATION OF AN ARABIDOPSIS-LIPOXYGENASE GENE RESPONSIVE TO METHYL JASMONATE AND WOUNDING [J].
BELL, E ;
MULLET, JE .
PLANT PHYSIOLOGY, 1993, 103 (04) :1133-1137
[7]  
BJORKMAN T, 1991, PLANTA, V185, P379
[8]   New evidence about the relationship between water channel activity and calcium in salinity-stressed pepper plants [J].
Cabañero, FJ ;
Martínez-Ballesta, MC ;
Teruel, JA ;
Carvajal, M .
PLANT AND CELL PHYSIOLOGY, 2006, 47 (02) :224-233
[9]   Phosphatidic acid accumulation is an early response in the Cf-4/Avr4 interaction [J].
de Jong, CF ;
Laxalt, AM ;
Bargmann, BOR ;
de Wit, PJGM ;
Joosten, MHAJ ;
Munnik, T .
PLANT JOURNAL, 2004, 39 (01) :1-12
[10]   Herbivore-induced resistance against microbial pathogens in Arabidopsis [J].
De Vos, Martin ;
Van Zaanen, Wendy ;
Koornneef, Annemart ;
Korzelius, Jerome P. ;
Dicke, Marcel ;
Van Loon, L. C. ;
Pieterse, Corne M. J. .
PLANT PHYSIOLOGY, 2006, 142 (01) :352-363