Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation

被引：24

作者：

Nasonkin, Igor O.

Koliatsos, Vassilis E.

机构：

[1] Dept Pathol, Div Neuropathol, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pathol Neuropathol Neurol Neurosci & Psychia, Baltimore, MD 21205 USA

来源：

EXPERIMENTAL NEUROLOGY | 2006年 / 201卷 / 02期

关键词：

human embryonic stem cells; neural differentiation; transplantation; immunogenicity; sialic; Neu5Gc;

D O I：

10.1016/j.expneurol.2006.05.002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A concern recently has been raised that human embryonic stein cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium. (c) 2006 Elsevier Inc. All rights reserved.

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收藏

页码：525 / 529

页数：5

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