Lyngbyatoxin biosynthesis: Sequence of biosynthetic gene cluster and identification of a novel aromatic prenyltransferase

被引:171
作者
Edwards, DJ [1 ]
Gerwick, WH [1 ]
机构
[1] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA
关键词
D O I
10.1021/ja047876g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The lyngbyatoxins are potent skin irritants produced by Lyngbya majuscula and cause a condition known as "Swimmer's Itch" off Honolulu, HI. Reported is the molecular cloning of the lyngbyatoxin (ltx) biosynthetic gene cluster from L. majuscula using a strategy based on its predicted nonribosomal peptide synthetase (NRPS) assembly. The biosynthetic gene cluster spans 11.3 kilobase pairs and encodes for a two-module NRPS (LtxA), a P450 monooxygenase (LtxB), an aromatic prenyltransferase (LtxC), and an oxidase/reductase protein (LtxD). LtxC was heterologously produced and purified from E. coli and shown to catalyze the transfer of a geranyl group to (-)-indolactam V as the final step in the biosynthesis of lyngbyatoxin A. Copyright © 2003 American Chemical Society.
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页码:11432 / 11433
页数:2
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