Sulfation of (1→3)-β-D-glucan from the fruiting bodies of Russula virescens and antitumor activities of the modifiers

A water-insoluble (1 -> 3)-beta-D-glucan isolated from the fresh fruiting bodies of Russula virescens was sulfated using sulfur trioxide-pyridine complex as reagent in dimethyl sulfoxide. Depending on the reaction conditions, the products showed different degrees of sulfation (DS) ranging from 0.17 to 1.17 and different weight average molecular weights (M(w)s) ranging from 2.5 X 10(4) to 1.2 x 10(5) Da. Moreover, the antitumor activities of the five sulfated derivatives against Sarcoma 180 tumor cell were tested both in vitro and in vivo. The results indicated that the native (1 -> 3)-beta-D-glucan did not show antitumor activity, while the sulfated derivatives exhibited enhanced antitumor activities. This study demonstrated that DS and M, could influence the antitumor activities of the sulfated derivatives. (C) 2009 Elsevier Ltd. All rights reserved.