Prospecting for novel lipase genes using PCR

被引：97

作者：

Bell, PJL ^{[1
]}

Sunna, A

Gibbs, MD

Curach, NC

Nevalainen, H

Bergquist, PL

机构：

[1] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia

[2] Univ Potsdam, D-14476 Golm, Germany

[3] Univ Auckland, Sch Med, Div Mol Med, Auckland, New Zealand

来源：

MICROBIOLOGY-SGM | 2002年 / 148卷

关键词：

triacylglycerol hydrolase; biomass; thermophilic; environment;

D O I：

10.1099/00221287-148-8-2283

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the alpha/beta hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.

引用

页码：2283 / 2291

页数：9