Drosophila Tempura, a Novel Protein Prenyltransferase α Subunit, Regulates Notch Signaling Via Rab1 and Rab11

被引：44

作者：

Charng, Wu-Lin ^{[1
]}

Yamamoto, Shinya ^{[1
,2
,3
]}

Jaiswal, Manish ^{[2
,4
]}

Bayat, Vafa ^{[1
,5
]}

Xiong, Bo ^{[1
]}

Zhang, Ke ^{[6
]}

Sandoval, Hector ^{[2
]}

David, Gabriela ^{[1
]}

Gibbs, Stephen ^{[2
]}

Lu, Hsiang-Chih ^{[1
]}

Chen, Kuchuan ^{[1
]}

Giagtzoglou, Nikos ^{[2
,7
]}

Bellen, Hugo J. ^{[1
,2
,3
,4
,6
,8
]}

机构：

[1] Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[3] Texas Childrens Hosp, Jan & Dan Duncan Neurol Res Inst, Houston, TX 77030 USA

[4] Baylor Coll Med, Howard Hughes Med Inst, Houston, TX 77030 USA

[5] Baylor Coll Med, Med Scientist Training Program, Houston, TX 77030 USA

[6] Baylor Coll Med, Program Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA

[7] Baylor Coll Med, Dept Neurol, Houston, TX 77030 USA

[8] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA

来源：

PLOS BIOLOGY | 2014年 / 12卷 / 01期

关键词：

SENSORY ORGAN DEVELOPMENT; DAUGHTER CELL FATES; GERANYLGERANYL TRANSFERASE; ASYMMETRIC DIVISION; CDNA CLONING; COMPONENT-A; GENE; MEMBRANE; DELTA; TRAFFICKING;

D O I：

10.1371/journal.pbio.1001777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-alpha-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling.

引用

页数：16

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