Promotion of neutrophil adherence to human osteoblasts by microcrystals and f-Met-Leu-Phe

被引:6
作者
Bouchard, L [1 ]
Naccache, PH [1 ]
Poubelle, PE [1 ]
机构
[1] Univ Laval, Ctr Rech Rhumatol & Immunol, Dept Med, Laval, PQ, Canada
基金
加拿大健康研究院;
关键词
arthritis; bone resorption; infection; cytokines; neutrophils; adhesion;
D O I
10.1016/S0006-291X(02)00911-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human osteoblast-like cells (hOB) stimulated by monosodium urate monohydrate (MSUM) or calcium pyrophosphate dihydrate (CPPD) microcrystals produce the neutrophil chemoattractant IL-8. We investigated whether human neutrophils can adhere to hOB and respond to hOB preactivated by MSUM, CPPD, or by f-Met-Leu-Phe (fMLP). Confluent hOB were coincubated with human blood neutrophils in the presence of MSUM, CPPD or fMLP. MSUM, CPPD, and fMLP stimulated a significant adherence of neutrophils to hOB after a I h incubation. This effect was not abrogated by pretreating the cells with an anti-CD18 mAb. MSUM stimulated more efficiently the adherence of neutrophils to non-preactivated hOB while CPPD were more efficient when hOB were preactivated. Crystal-free conditioned media from MSUM- or CPPD-stimulated hOB mobilized intracellular free calcium in human neutrophils. Thus, microcrystals were powerful promoters of neutrophil adherence to hOB via a CD18-independent mechanism. The bacterial peptide fMLP also stimulated the adherence of neutrophils to hOB. Functional neutrophil-hOB interactions could be important in bone pathophysiology of crystal- or infection-associated arthritis. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:759 / 764
页数:6
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