Transdifferentiation potential of human mesenchymal stem cells derived from bone marrow

被引:297
作者
Song, L [1 ]
Tuan, RS [1 ]
机构
[1] NIAMSD, Cartilage & Orthopaed Branch, NIH, US Dept HHS, Bethesda, MD 20892 USA
关键词
plasticity; osteogenesis; adipogenesis; chondrogenesis;
D O I
10.1096/fj.03-1100fje
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transdifferentiation is a process whereby one cell type committed to and progressing along a specific developmental lineage switches into another cell type of a different lineage through genetic reprogramming. Even though this process has been well studied and established in amphibian systems, it is unclear if mammalian cells possess the same potential. Recent in vivo transplantation studies showed that adult mesenchymal stem cells (MSCs) were able to differentiate into mesoderm-derived cell types as well as cells with neuroectodermal and endodermal characteristics, suggesting that transdifferentiation occurs in mammalian systems. However, there are concerns over these findings because of the possibility of progenitor cell contamination and cell fusion. In this study, we have developed an in vitro differentiation strategy to assess if human MSCs that have differentiated into a given mesenchyme cell lineage can transdifferentiate into other cell types in response to inductive extracellular cues. Our results showed that fully differentiated cells from hMSCs were capable of dedifferentiation and transdifferentiation into cells of another developmental lineage at single cell levels.
引用
收藏
页码:980 / +
页数:15
相关论文
共 32 条
[31]   Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats [J].
Zhao, LR ;
Duan, WM ;
Reyes, M ;
Keene, CD ;
Verfaillie, CM ;
Low, WC .
EXPERIMENTAL NEUROLOGY, 2002, 174 (01) :11-20
[32]   Human adipose tissue is a source of multipotent stem cells [J].
Zuk, PA ;
Zhu, M ;
Ashjian, P ;
De Ugarte, DA ;
Huang, JI ;
Mizuno, H ;
Alfonso, ZC ;
Fraser, JK ;
Benhaim, P ;
Hedrick, MH .
MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (12) :4279-4295