Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae

被引：28

作者：

Mandrioli, M ^{[1
]}

机构：

[1] Univ Modena, Dipartimento Biol Anim, I-41100 Modena, Italy

来源：

CHROMOSOME RESEARCH | 2002年 / 10卷 / 04期

关键词：

Bal31; holocentric chromosome; Lepidoptera; Mamestra brassicae; telomere; TRAS1;

D O I：

10.1023/A:1016515607278

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Telomeres of the Mamestra brassica holocentric chromosomes were studied by Southern blotting, in-situ hybridization and Bal31 assay evidencing the presence of the telomeric (TTAGG)(n) repeat. Successively, molecular analysis of telomeres showed that TRAS1 transposable elements were present at the subtelomeric regions of autosomes but not in the NOR-bearing telomeres of the Z and W sex chromosomes. TRAS1 appeared to be transcriptionally active and non-methylated, as evaluated by RT-PCR and digestion with MspI and HpaII. Finally, dot-blotting experiments showed that the 2.8 +/- 0.5% of the M. brassicae genome consists of TRAS1.

引用

页码：279 / 286

页数：8

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