Influence of the stiffness of three-dimensional alginate/collagen-I interpenetrating networks on fibroblast biology

被引:232
作者
da Cunha, Cristiana Branco [1 ,2 ,3 ,4 ,5 ]
Klumpers, Darinka D. [1 ,2 ,6 ]
Li, Weiwei A. [1 ,2 ]
Koshy, Sandeep T. [1 ,2 ,7 ]
Weaver, James C. [2 ]
Chaudhuri, Ovijit [8 ]
Granja, Pedro L. [4 ,9 ,10 ]
Mooney, David J. [1 ,2 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA
[3] Inst Mol Pathol & Immunol Univ Porto IPATIMUP, P-4200465 Porto, Portugal
[4] Inst Engn Biomed Univ Porto INEB, P-4150180 Porto, Portugal
[5] Fac Med Univ Porto, Hosp S Joao, P-4200319 Porto, Portugal
[6] Vrije Univ Amsterdam Med Ctr, Res Inst MOVE, Dept Orthoped Surg, NL-1081 HV Amsterdam, Netherlands
[7] Harvard Mit Div Hlth Sci & Technol, Cambridge, MA USA
[8] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA
[9] Fac Engn Univ Porto, Dept Met & Mat Engn, P-4200465 Porto, Portugal
[10] Inst Ciencias Biomed Abel Salazar Univ Porto ICBA, P-4050313 Porto, Portugal
基金
美国国家科学基金会;
关键词
Wound healing; ECM (extracellular matrix); Mechanical properties; Interpenetrating networks (IPNs); Inflammation; Wound dressing biomaterial; EXTRACELLULAR-MATRIX; MECHANICAL-PROPERTIES; DELIVERY-SYSTEMS; COLLAGEN GELS; TISSUE; HYDROGELS; CONTRACTION; ALGINATE; ADHESION; MECHANOTRANSDUCTION;
D O I
10.1016/j.biomaterials.2014.06.047
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Wound dressing biomaterials are increasingly being designed to incorporate bioactive molecules to promote healing, but the impact of matrix mechanical properties on the biology of resident cells orchestrating skin repair and regeneration remains to be fully understood. This study investigated whether tuning the stiffness of a model wound dressing biomaterial could control the behavior of dermal fibroblasts. Fully interpenetrating networks (IPNs) of collagen-I and alginate were fabricated to enable gel stiffness to be tuned independently of gel architecture, polymer concentration or adhesion ligand density. Three-dimensional cultures of dermal fibroblasts encapsulated within matrices of different stiffness were shown to promote dramatically different cell morphologies, and enhanced stiffness resulted in upregulation of key-mediators of inflammation such as IL-10 and COX-2. These findings suggest that simply modulating the matrix mechanical properties of a given wound dressing biomaterial deposited at the wound site could regulate the progression of wound healing. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:8927 / 8936
页数:10
相关论文
共 63 条
[1]
Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation [J].
Achilli, Matteo ;
Mantovani, Diego .
POLYMERS, 2010, 2 (04) :664-680
[2]
Fibrin-polyethylene oxide interpenetrating polymer networks: New self-supported biomaterials combining the properties of both protein gel and synthetic polymer [J].
Akpalo, E. ;
Bidault, L. ;
Boissiere, M. ;
Vancaeyzeele, C. ;
Fichet, O. ;
Larreta-Garde, V. .
ACTA BIOMATERIALIA, 2011, 7 (06) :2418-2427
[3]
Size-Dependent Rheology of Type-I Collagen Networks [J].
Arevalo, Richard C. ;
Urbach, Jeffrey S. ;
Blair, Daniel L. .
BIOPHYSICAL JOURNAL, 2010, 99 (08) :L65-L67
[4]
LIVING TISSUE FORMED INVITRO AND ACCEPTED AS SKIN-EQUIVALENT TISSUE OF FULL THICKNESS [J].
BELL, E ;
EHRLICH, HP ;
BUTTLE, DJ ;
NAKATSUJI, T .
SCIENCE, 1981, 211 (4486) :1052-1054
[5]
IL-10 is a central regulator of cyclooxygenase-2 expression and prostaglandin production [J].
Berg, DJ ;
Zhang, J ;
Lauricella, DM ;
Moore, SA .
JOURNAL OF IMMUNOLOGY, 2001, 166 (04) :2674-2680
[6]
Wound healing dressings and drug delivery systems: A review [J].
Boateng, Joshua S. ;
Matthews, Kerr H. ;
Stevens, Howard N. E. ;
Eccleston, Gillian M. .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2008, 97 (08) :2892-2923
[7]
Mechanical regulation of vascular growth and tissue regeneration in vivo [J].
Boerckel, Joel D. ;
Uhrig, Brent A. ;
Willett, Nick J. ;
Huebsch, Nathaniel ;
Guldberg, Robert E. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (37) :E674-E680
[8]
Chaudhuri O, 2014, NAT MAT
[9]
Reversible stress softening of actin networks [J].
Chaudhuri, Ovijit ;
Parekh, Sapun H. ;
Fletcher, Daniel A. .
NATURE, 2007, 445 (7125) :295-298
[10]
Tissue engineering for cutaneous wounds [J].
Clark, Richard A. F. ;
Ghosh, Kaustabh ;
Tonnesen, Marcia G. .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2007, 127 (05) :1018-1029