Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development

被引：29

作者：

Zhang, Youjun ^{[1
,2
]}

Sun, Feng ^{[2
]}

Fettke, Joerg ^{[3
]}

Schoettler, Mark Aurel ^{[1
]}

Ramsden, Lawrence ^{[2
]}

Fernie, Alisdair R. ^{[1
]}

Lim, Boon Leong ^{[2
]}

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

[2] Univ Hong Kong, Sch Biol Sci, Pokfulam, Hong Kong, Peoples R China

[3] Univ Potsdam, Inst Biochem & Biol, Golm, Germany

来源：

FEBS LETTERS | 2014年 / 588卷 / 20期

关键词：

Potato; AtPAP2; Photosynthesis; Tuber yield; Sugar efflux; SUCROSE-PHOSPHATE SYNTHASE; PURPLE ACID-PHOSPHATASE; PROTEIN; OVEREXPRESSION; PHOTOSYNTHESIS; MEMBRANE; GROWTH; YIELD; LEAF;

D O I：

10.1016/j.febslet.2014.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in carbon flow and sink/source activities can affect floral, architectural, and reproductive traits of plants. In potato, overexpression (OE) of the purple acid phosphatase 2 of Arabidopsis (AtPAP2) resulted in earlier flowering, faster growth rate, increased tubers and tuber starch content, and higher photosynthesis rate. There was a significant change in sucrose, glucose and fructose levels in leaves, phloem and sink biomass of the OE lines, consistent with an increased expression of sucrose transporter 1 (StSUT1). Furthermore, the expression levels and enzyme activity of sucrose-phosphate synthase (SPS) were also significantly increased in the OE lines. These findings strongly suggest that higher carbon supply from the source and improved sink strength can improve potato tuber yield. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3726 / 3731

页数：6

共 26 条

[1] Carbohydrate Export from the Leaf: A Highly Regulated Process and Target to Enhance Photosynthesis and Productivity [J].

Ainsworth, Elizabeth A. ;

Bush, Daniel R. .

PLANT PHYSIOLOGY, 2011, 155 (01) :64-69

[2] Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants [J].

Baroja-Fernández, E ;

Muñoz, FJ ;

Saikusa, T ;

Rodríguez-López, M ;

Akazawa, T ;

Pozueta-Romero, J .

PLANT AND CELL PHYSIOLOGY, 2003, 44 (05) :500-509

[3] Elevated sucrose-phosphate synthase activity in transgenic tobacco sustains photosynthesis in older leaves and alters development [J].

Baxter, CJ ;

Foyer, CH ;

Turner, J ;

Rolfe, SA ;

Quick, WP .

JOURNAL OF EXPERIMENTAL BOTANY, 2003, 54 (389) :1813-1820

[4] Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants:: an efficient protocol of transformation [J].

Beaujean, A ;

Sangwan, RS ;

Lecardonnel, A ;

Sangwan-Norreel, BS .

JOURNAL OF EXPERIMENTAL BOTANY, 1998, 49 (326) :1589-1595

[5] Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber [J].

Chakraborty, Subhra ;

Chakraborty, Niranjan ;

Agrawal, Lalit ;

Ghosh, Sudip ;

Narula, Kanika ;

Shekhar, Shubhendu ;

Naik, Prakash S. ;

Pande, P. C. ;

Chakrborti, Swarup Kumar ;

Datta, Asis .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (41) :17533-17538

[6] Sucrose transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response [J].

Chincinska, Izabela A. ;

Liesche, Johannes ;

Kruegel, Undine ;

Michalska, Justyna ;

Geigenberger, Peter ;

Grimm, Bernhard ;

Kuehn, Christina .

PLANT PHYSIOLOGY, 2008, 146 (02) :515-528

[7] Specific binding of vf14-3-3a isoform to the plasma membrane H+-ATPase in response to blue light and fusicoccin in guard cells of broad bean [J].

Emi, T ;

Kinoshita, T ;

Shimazaki, K .

PLANT PHYSIOLOGY, 2001, 125 (02) :1115-1125

[8] Fructose 2,6-bisphosphate activates pyrophosphate: fructose-6-phosphate 1-phosphotransferase and increases triose phosphate to hexose phosphate cycling in heterotrophic cells [J].

Fernie, AR ;

Roscher, A ;

Ratcliffe, RG ;

Kruger, NJ .

PLANTA, 2001, 212 (02) :250-263

[9] ROLE OF SUCROSE-PHOSPHATE SYNTHASE IN SUCROSE METABOLISM IN LEAVES [J].

HUBER, SC ;

HUBER, JL .

PLANT PHYSIOLOGY, 1992, 99 (04) :1275-1278

[10] Simultaneous boosting of source and sink capacities doubles tuber starch yield of potato plants [J].

Jonik, Claudia ;

Sonnewald, Uwe ;

Hajirezaei, Mohammad-Reza ;

Fluegge, Ulf-Ingo ;

Ludewig, Frank .

PLANT BIOTECHNOLOGY JOURNAL, 2012, 10 (09) :1088-1098

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