Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development

被引：29

作者：

Zhang, Youjun ^{[1
,2
]}

Sun, Feng ^{[2
]}

Fettke, Joerg ^{[3
]}

Schoettler, Mark Aurel ^{[1
]}

Ramsden, Lawrence ^{[2
]}

Fernie, Alisdair R. ^{[1
]}

Lim, Boon Leong ^{[2
]}

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

[2] Univ Hong Kong, Sch Biol Sci, Pokfulam, Hong Kong, Peoples R China

[3] Univ Potsdam, Inst Biochem & Biol, Golm, Germany

来源：

FEBS LETTERS | 2014年 / 588卷 / 20期

关键词：

Potato; AtPAP2; Photosynthesis; Tuber yield; Sugar efflux; SUCROSE-PHOSPHATE SYNTHASE; PURPLE ACID-PHOSPHATASE; PROTEIN; OVEREXPRESSION; PHOTOSYNTHESIS; MEMBRANE; GROWTH; YIELD; LEAF;

D O I：

10.1016/j.febslet.2014.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in carbon flow and sink/source activities can affect floral, architectural, and reproductive traits of plants. In potato, overexpression (OE) of the purple acid phosphatase 2 of Arabidopsis (AtPAP2) resulted in earlier flowering, faster growth rate, increased tubers and tuber starch content, and higher photosynthesis rate. There was a significant change in sucrose, glucose and fructose levels in leaves, phloem and sink biomass of the OE lines, consistent with an increased expression of sucrose transporter 1 (StSUT1). Furthermore, the expression levels and enzyme activity of sucrose-phosphate synthase (SPS) were also significantly increased in the OE lines. These findings strongly suggest that higher carbon supply from the source and improved sink strength can improve potato tuber yield. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3726 / 3731

页数：6

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