Characterization of the last step of lignin blosynthesis in Zinnia elegans suspension cell cultures

被引：35

作者：

Gabaldon, Carlos

Lopez-Serrano, Matias

Pomar, Federico

Merino, Fuencisla

Cuello, Juan

Pedreno, M. A.

Barcelo, A. Ros ^{[1
]}

机构：

[1] Univ Murcia, Dept Plant Biol, E-30100 Murcia, Spain

[2] CIA Mabegondo, E-15080 La Coruna, Spain

[3] Univ A Coruna, Dept Plant Biol, E-15071 La Coruna, Spain

来源：

FEBS LETTERS | 2006年 / 580卷 / 18期

关键词：

lignin; monolignols; peroxidase; suspension cell; cultures; Zinnia;

D O I：

10.1016/j.febslet.2006.06.088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The last step of lignin biosynthesis in Zinnia elegans suspension cell cultures (SCCs) catalyzed by peroxidase (ZePrx) has been characterized. The k(3) values shown by ZePrx for the three monolignols revealed that sinapyl alcohol was the best substrate, and were proportional to their oxido/reduction potentials, signifying that these reactions are driven exclusively by redox thermodynamic forces. Feeding experiments demonstrate that cell wall lignification in SCCs is controlled by the rate of supply of H2O2. The results also showed that sites for monolignol beta-O-4 cross-coupling in cell walls may be saturated, suggesting that the growth of the lineal lignin macromolecule is not infinite. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4311 / 4316

页数：6

共 29 条

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