In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats

被引：57

作者：

Shiota, G ^{[1
]}

Kunisada, T

Oyama, K

Udagawa, A

Nomi, T

Tanaka, K

Tsutsumi, A

Isono, M

Nakamura, T

Hamada, H

Sakatani, T

Sell, S

Sato, K

Ito, H

Kawasaki, H

机构：

[1] Tottori Univ, Fac Med, Dept Internal Med 2, Yonago, Tottori 6838504, Japan

[2] Tottori Univ, Fac Med, Dept Immunol, Yonago, Tottori 6838504, Japan

[3] Tottori Univ, Fac Med, Dept Mol Biol, Yonago, Tottori 6838504, Japan

[4] Japanese Fdn Canc Res, Ctr Canc Chemotherapy, Tokyo 170, Japan

[5] Tottori Univ, Fac Med, Dept Pathol 1, Yonago, Tottori 6838504, Japan

[6] Albany Med Coll, Dept Pathol, Albany, NY USA

来源：

FEBS LETTERS | 2000年 / 470卷 / 03期

关键词：

hepatocyte growth factor; oval cell; Solt-Farber model; liver regeneration;

D O I：

10.1016/S0014-5793(00)01337-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, rye transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats mere infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats. (C) 2000 Federation of European Biochemical Societies.

引用

页码：325 / 330

页数：6

共 32 条

[1] Murine cytomegalovirus immediate-early promoter directs astrocyte-specific expression in transgenic mice [J].

Aiba-Masago, S ;

Baba, S ;

Li, RY ;

Shinmura, Y ;

Kosugi, I ;

Arai, Y ;

Nishimura, M ;

Tsutsui, Y .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (03) :735-743

[2] Hepatic stem cells [J].

Alison, M ;

Sarraf, C .

JOURNAL OF HEPATOLOGY, 1998, 29 (04) :676-682

[3] Liver stem cells: when the going gets tough they get going [J].

Alison, MR ;

Golding, M ;

Sarraf, CE .

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 1997, 78 (06) :365-381

[4] DIFFERENTIAL TUBULOGENIC AND BRANCHING MORPHOGENETIC ACTIVITIES OF GROWTH-FACTORS - IMPLICATIONS FOR EPITHELIAL TISSUE-DEVELOPMENT [J].

BARROS, EJG ;

SANTOS, OFP ;

MATSUMOTO, K ;

NAKAMURA, T ;

NIGAM, SK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4412-4416

[5] Expression of the stem cell factor receptor c-kit in normal and diseased pediatric liver:: Identification of a human hepatic progenitor cell? [J].

Baumann, U ;

Crosby, HA ;

Ramani, P ;

Kelly, DA ;

Strain, AJ .

HEPATOLOGY, 1999, 30 (01) :112-117

[6]

DUNSFORD HA, 1989, CANCER RES, V49, P4887

[7] A PRECURSOR PRODUCT RELATIONSHIP EXISTS BETWEEN OVAL CELLS AND HEPATOCYTES IN RAT-LIVER [J].

EVARTS, RP ;

NAGY, P ;

MARSDEN, E ;

THORGEIRSSON, SS .

CARCINOGENESIS, 1987, 8 (11) :1737-1740

[8] Hepatocyte differentiation and liver progenitor cells [J].

Fausto, N. .

CURRENT OPINION IN CELL BIOLOGY, 1990, 2 (06) :1036-1042

[9] HEPATOCYTE GROWTH-FACTOR INDUCES PROLIFERATION AND DIFFERENTIATION OF MULTIPOTENT AND ERYTHROID HEMATOPOIETIC PROGENITORS [J].

GALIMI, F ;

BAGNARA, GP ;

BONSI, L ;

COTTONE, E ;

FOLLENZI, A ;

SIMEONE, A ;

COMOGLIO, PM .

JOURNAL OF CELL BIOLOGY, 1994, 127 (06) :1743-1754

[10]

Grisham J. W., 1997, P233, DOI 10.1016/B978-012563455-7/50009-X

← 1 2 3 4 →