In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats

被引：57

作者：

Shiota, G ^{[1
]}

Kunisada, T

Oyama, K

Udagawa, A

Nomi, T

Tanaka, K

Tsutsumi, A

Isono, M

Nakamura, T

Hamada, H

Sakatani, T

Sell, S

Sato, K

Ito, H

Kawasaki, H

机构：

[1] Tottori Univ, Fac Med, Dept Internal Med 2, Yonago, Tottori 6838504, Japan

[2] Tottori Univ, Fac Med, Dept Immunol, Yonago, Tottori 6838504, Japan

[3] Tottori Univ, Fac Med, Dept Mol Biol, Yonago, Tottori 6838504, Japan

[4] Japanese Fdn Canc Res, Ctr Canc Chemotherapy, Tokyo 170, Japan

[5] Tottori Univ, Fac Med, Dept Pathol 1, Yonago, Tottori 6838504, Japan

[6] Albany Med Coll, Dept Pathol, Albany, NY USA

来源：

FEBS LETTERS | 2000年 / 470卷 / 03期

关键词：

hepatocyte growth factor; oval cell; Solt-Farber model; liver regeneration;

D O I：

10.1016/S0014-5793(00)01337-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, rye transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats mere infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats. (C) 2000 Federation of European Biochemical Societies.

引用

页码：325 / 330

页数：6

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