Identification of a pH regulated Na+/H+ antiporter of Methanococcus jannaschii

被引：20

作者：

Hellmer, J ^{[1
]}

Pätzold, R ^{[1
]}

Zeilinger, C ^{[1
]}

机构：

[1] Univ Hannover, Inst Biophys, D-30419 Hannover, Germany

来源：

FEBS LETTERS | 2002年 / 527卷 / 1-3期

关键词：

Na+/H+ antiporter; pH regulation; Methanococcus jannaschii;

D O I：

10.1016/S0014-5793(02)03244-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na+/H+ antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na+ flux in everted vesicles. From the tested clones only Mj0057 displayed Na+ (Li+)/H+ antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K-m values for Na+ and Li+ were approximately 10 and 2.5 mM, respectively. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：245 / 249

页数：5