Improvement of enterocin P purification process

被引：6

作者：

Cuozzo, S. ^{[1
]}

Calvez, S. ^{[1
]}

Prevost, H. ^{[1
]}

Drider, D. ^{[1
]}

机构：

[1] Ecole Natl Ingn Ind Tech Agricoles & Alimentaires, Lab Microbiol Alimentaire & Ind, F-82225 Nantes 3, France

来源：

FOLIA MICROBIOLOGICA | 2006年 / 51卷 / 05期

关键词：

D O I：

10.1007/BF02931583

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purification and heterologous expression of enterocin P (EntP), a sec-dependent bacteriocin produced by Enterococcus faecium, in Escherichia coli is described. PCR-amplified product of the enterocin P structural gene entP was cloned into plasmid pET-32b under the control of the inducible T7lac promoter. The neo-synthesized EntP was genetically modified by an addition of 3 extra amino acids, leading to recombinant EntRP. Active EntRP was recovered from the cytoplasmic soluble fraction of E. coli harboring appropriate recombinant plasmid, characterized by ELISA and Western-blot analysis and purified by immunoaffinity chromatography. The use of E. coli as heterologous host and pET-32b as expressing vector offers promising tools for heterologous production of class IIa bacteriocin.

引用

页码：401 / 405

页数：5

共 28 条

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