Characterization of the second form of NADH-cytochrome b5 reductase gene from arachidonic acid-producing fungus Mortierella alpina 1S-4

被引：13

作者：

Certik, M ^{[1
]}

Sakuradani, E ^{[1
]}

Kobayashi, M ^{[1
]}

Shimizu, S ^{[1
]}

机构：

[1] Kyoto Univ, Grad Shc Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 88卷 / 06期

关键词：

cytochrome b(5) reductase; cloning; Mortierella fungus;

D O I：

10.1016/S1389-1723(00)87098-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The second type of cytochrome b(5) reductase (Cb5R-II) gene was characterized in the arachidonic acid-producing fungus Mortierella alpina 1S-4. Its cDNA (897 bp) and predicted amino acid (298 as) sequences show more than 70% similarity to the previously isolated first type of Cb5R. Highly conserved exon-intron organization suggests that the two genes evolved from the duplication of a common ancestral gene. Cb5R-II has a flavin-binding domain at its highly hydrophobic N-terminal and an NADH-binding domain at the C-terminal. In comparison with Cb5R genes from other sources, high homology (46-54%) was found for yeast and plant genes. Phylogenetic analysis revealed that microbial and plant Cb5R genes represent a gene family evolved from one prototype and are different from mammalian Cb5R genes.

引用

页码：667 / 671

页数：5

共 23 条

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