Syndecan-2 overexpression regulates adhesion and migration through cooperation with integrin α2

被引：48

作者：

Choi, Sojoong ^{[2
,3
]}

Kim, Yeonhee ^{[2
,3
]}

Park, Haein ^{[2
,3
]}

Han, Inn-Oc ^{[4
]}

Chung, Eunkyung ^{[5
]}

Lee, Sung-Yul ^{[6
]}

Kim, Yong-Bae ^{[6
]}

Lee, Jung Weon ^{[6
]}

Oh, Eok-Soo ^{[2
,3
]}

Yi, Jae Youn ^{[1
]}

机构：

[1] Korea Inst Radiol & Med Sci, Lab Modulat Radiobiol Responses, Seoul 139706, South Korea

[2] Ewha Womans Univ, Dept Life Sci, Div Life & Pharmaceut Sci, Seoul 120750, South Korea

[3] Ewha Womans Univ, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South Korea

[4] Inha Univ, Coll Med, Dept Physiol & Biophys, Inchon 402751, South Korea

[5] Hallym Univ, Dept Biomed Sci, Chunchon 200702, South Korea

[6] Seoul Natl Univ, Canc Res Inst, Coll Med, Dept Tumor Biol & Mol & Clin Oncol, Seoul 110799, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 384卷 / 02期

关键词：

Syndecan-2; Integrin alpha 2; Adhesion; Migration; Invasion; Colon cancer cell; COLON-CARCINOMA CELLS; TUMORIGENIC ACTIVITY; PROTEIN-KINASE; CANCER-CELLS; EPITHELIAL-CELLS; EXPRESSION; COLLAGEN; ALPHA-5-BETA-1; ACTIVATION; PATHWAY;

D O I：

10.1016/j.bbrc.2009.04.093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Syndecan-2, a transmembrane heparan Sulfate proteoglycan, is known to serve as an adhesion receptor, but details of the regulatory mechanism governing syndecan-2 cell adhesion and migration remain unclear. Here, we examined this regulatory mechanism, showing that overexpression of syndecan-2 enhanced collagen adhesion, cell migration and invasion of normal rat intestinal epithelial cells (RIE1), and increased integrin of alpha 2 expression levels. Interestingly, RIE1 cells transfected with either syndecan-2 or integrin of alpha 2 showed similar adhesion and migration patterns, and a function-blocking anti-integrin alpha 2 antibody abolished syndecan-2-mediated adhesion and migration. Consistent with these findings, transfection of integrin alpha 2 siRNA diminished syndecan-2-induced cell migration in HCT116 human colon cancer cells. Taken together. these results demonstrate a novel cooperation between syndecan-2 and integrin alpha 2 beta 1 in adhesion-mediated cell migration and invasion. This interactive dynamic might be a possible mechanism Underlying the tumorigenic activities of colon cancer cells. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：231 / 235

页数：5

共 24 条

[11] α5β1 integrin protects intestinal epithelial cells from apoptosis through a phosphatidylinositol 3-kinase and protein kinase B-dependent pathway [J].