The use of ferromagnetic Dacron as solid-phase in chemiluminescent assays

被引:8
作者
Pinheiro, SMB
Carvalho, LB [1 ]
Chaves, MEC
机构
[1] Univ Fed Pernambuco, Dept Bioquim, BR-50670910 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Lab Imumopatol Keizo Asami LIKA, BR-50670910 Recife, PE, Brazil
关键词
chemiluminescence; Dacron; ferromagnetic Dacron; human serum albumin; magnetic particles;
D O I
10.1023/A:1008963202258
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Ferromagnetic Dacron hydrazide particles (63-74 mu m) were activated either by converting hydrazide groups to azide or binding glutaraldehyde to the hydrazide groups. Human serum albumin (HSA) was immobilised to the activated matrices and antibodies against HSA were detected by using the chemiluminescent assay. The use of these particles (either Dacron-azide or Dacron-glutaraldehyde) gave similar results compared with the paramagnetic particles commercially available from Ciba Corning.
引用
收藏
页码:919 / 922
页数:4
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