Progress and prospects: techniques for site-directed mutagenesis in animal models

被引：17

作者：

Yan, Z. ^{[1
,2
]}

Sun, X. ^{[1
,2
]}

Engelhardt, J. F. ^{[1
,2
]}

机构：

[1] Univ Iowa, Carver Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA

[2] Univ Iowa, Carver Coll Med, Ctr Gene Therapy, Iowa City, IA 52242 USA

来源：

GENE THERAPY | 2009年 / 16卷 / 05期

关键词：

gene targeting; recombinant adeno-associated virus; animal models; zinc-finger nucleases; homologous recombination; somatic cell nuclear transfer; ZINC-FINGER NUCLEASES; SPERMATOGONIAL STEM-CELLS; TARGETED MUTAGENESIS; MAMMALIAN-CELLS; CYSTIC-FIBROSIS; KNOCKOUT MICE; CFTR GENE; IN-VITRO; ZEBRAFISH; TRANSPLANTATION;

D O I：

10.1038/gt.2009.16

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the past 2 years, new gene-targeting approaches using adeno-associated virus and designer zinc-finger nucleases have been successfully applied to the production of genetically modified ferrets, pigs, mice and zebrafish. Gene targeting using these tools has been combined with somatic cell nuclear transfer and germ cell transplantation to generate gene-targeted animal models. These new technical advances, which do not require the generation of embryonic stem cell-derived chimeras, will greatly accelerate the production of non-mouse animal models for biomedical research. Gene Therapy (2009) 16, 581-588; doi: 10.1038/gt.2009.16; published online 19 February 2009

引用

页码：581 / 588

页数：8

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