Mapping of obesity QTLs in a cross between mouse lines divergently selected on fat content

被引:69
作者
Horvat, S
Bünger, L
Falconer, VM
Mackay, P
Law, A
Bulfield, G
Keightley, PD
机构
[1] Univ Edinburgh, Inst Cell Anim & Populat Biol, Edinburgh EH9 3JT, Midlothian, Scotland
[2] Roslin Inst, Div Mol Biol, Roslin EH25 9PS, Midlothian, Scotland
关键词
D O I
10.1007/s003350010002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A genome-wide quantitative trait locus (QTL) analysis was performed in a polygenic obesity mouse model resulting from a long-term selection experiment. The parental lines were outbred lines divergently selected for 53 generations for high-fat (fat, F line) or low-fat (lean, L line) percentage (fat%) that differed fivefold in fat% at 14 weeks of age. Pin Fz population of 436 mice was used for the QTL analysis with 71 markers distributed across the genome. The analysis revealed significant QTLs Fob1 (for F-line obesity QTL 1), Fob2, Fob3, and Fob4 on Chromosomes (Chrs) 2, 12, 15, and X, respectively. None of these QTLs map to regions of known single gene obesity mutations (Lep(ob), Lepr(db), Cpd(fat): A(y), tub), though they map to regions of previously described obesity QTLs and candidate genes. The effects of Fob1, Fob3, Fob4 were additive, and that of FobZ was dominant. Fob2 also showed a significant female-specific effect. Fob1, FobZ, Fob3, and Fob4 explained 4.9%, 19.5%, 14.3%, and 7.3% of the F, phenotypic variance for fat%, respectively. This study identified four loci that contributed to the response to divergent selection and control a significant proportion of the difference in obesity between the F and L lines.
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页码:2 / 7
页数:6
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