Cloning of the chrysanthemum UEP1 promoter and comparative expression in florets and leaves of Dendranthema grandiflora

被引：27

作者：

Annadana, S

Beekwilder, MJ

Kuipers, G

Visser, PB

Outchkourov, N

Pereira, A

Udayakumar, M

De Jong, J

Jongsma, MA

机构：

[1] Plant Res Int BV, NL-6700 AA Wageningen, Netherlands

[2] Univ Agr Sci Bangalore, Dept Crop Physiol, Bangalore 560065, Karnataka, India

来源：

TRANSGENIC RESEARCH | 2002年 / 11卷 / 04期

关键词：

chrysanthemum; florets; genetic engineering; GUS; molecular breeding; petal; transgene expression; ubiquitin extension protein;

D O I：

10.1023/A:1016313924844

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To attain high transgene expression in petal tissue of ray florets of chrysanthemum an endogenous ubiquitin extension protein (UEP1) promoter was cloned and tested with the beta-glucuronidase (GUS) reporter gene. Expression levels were compared with four heterologous promoters: chalcone synthase (chs-A) and zinc finger transcription factor (EPF2-5) from petunia, eceriferum (CER6) from Arabidopsis and multicystatin (PMC) from potato. The comparison of the expression levels of the different constructs in ray florets, disc florets, and leaves is presented. The highest mean expression in petal tissue of ray and disc florets was conferred by the UEP1 promoter, followed by CER6 and EPF2-5. The UEP1 promoter in ray florets confers over 50-fold enhancement in expression as compared to CaMV 35S-based promoters.

引用

页码：437 / 445

页数：9

共 27 条

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ACKERS CP, 1980, CAN J BOT, V58, P1000

[2]

ANDERSON NO, 1987, HORTSCIENCE, V22, P313

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