The interleukin-2 fusion protein, DAB(389)IL-2, inhibits the development of infectious virus in human immunodeficiency virus type 1-infected human peripheral blood mononuclear cells

被引：6

作者：

Zhang, LJ ^{[1
]}

Waters, CA ^{[1
]}

Poisson, LR ^{[1
]}

Estis, LF ^{[1
]}

Crumpacker, CS ^{[1
]}

机构：

[1] SERAGEN INC, HOPKINTON, MA USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1997年 / 175卷 / 04期

关键词：

D O I：

10.1086/513972

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

DAB(389)IL-2 is a genetically engineered fusion protein that reduces human immunodeficiency virus type 1 (HIV-1) replication in activated, interleukin (IL)-2 receptor (IL-2R)-expressing human peripheral blood mononuclear cells (PBMC). The level of infectious virus released by cultured PBMC after treatment with DAB(389)IL-2 was measured by a quantitative microculture assay. The inhibition of p24 antigen production was also evaluated in cultures that differed in duration of infection and activation state. Although the activation state of the cell and the time of DAB(389)IL-2 addition to the cultures influenced its anti-HIV activity, DAB(389)IL-2 treatment decreased levels of infectious HIV-1 to 0.1%-6.5% of untreated cell levels. DAB(389)IL-2 also decreased p24 antigen expression to 10%-48% of controls, even when added as late as 8 days after acute infection. Mutational variants of DAB(389)IL-2 that lack catalytic activity or IL-2R binding are without anti-HIV activity.

引用

页码：790 / 794

页数：5

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