Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor

被引：332

作者：

Zhang, FF

Wang, XL

Ai, SY

Sun, ZD

Wan, Q

Zhu, ZQ

Xian, YZ

Jin, LT ^{[1
]}

Yamamoto, K

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

[2] E China Normal Univ, Dept Elect Engn, Shanghai 200062, Peoples R China

[3] BAS Co Ltd, Sumida Ku, Tokyo 131, Japan

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 519卷 / 02期

基金：

中国国家自然科学基金;

关键词：

reagentless biosensor; ZnO nanorods; uricase; uric acid;

D O I：

10.1016/j.aca.2004.05.070

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85 degreesC and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 x 10(-6) to 1.0 x 10(-3) mol L-1 with a detection limit of 2.0 x 10(-6) mol L-1 at 3sigma. The apparent K-M(app) value for the uric acid sensor was estimated to be 0.238 mM, showing a high affinity. (C) 2004 Published by Elsevier B.V.

引用

页码：155 / 160

页数：6

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