Legionella phagosomes intercept vesicular traffic from endoplasmic reticulum exit sites

被引:367
作者
Kagan, JC [1 ]
Roy, CR [1 ]
机构
[1] Yale Univ, Sch Med, Boyer Ctr Mol Med, Sect Microbiol Pathogenesis, New Haven, CT 06536 USA
关键词
D O I
10.1038/ncb883
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It is unknown how Legionella pneumophila cells escape the degradative lysosomal pathway after phagocytosis by macrophages and replicate in an organelle derived from the endoplasmic reticulum. Here we show that, after internalization, L. pneumophila-containing phagosomes recruit early secretory vesicles. Once L. pneumophila phagosomes have intercepted early secretory vesicles they begin to acquire proteins residing in transitional and rough endoplasmic reticulum. The functions of Sar1 and ADP-ribosylation factor-1 are important for biogenesis of the L. pneumophila replicative organelle. These data indicate that L. pneumophila intercepts vesicular traffic from endoplasmic-reticulum exit sites to create an organelle that permits intracellular replication and prevents destruction by the host cell.
引用
收藏
页码:945 / 954
页数:10
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