Proof for a nonproteinaceous calcium-selective channel in Escherichia coli by total synthesis from (R)-3-hydroxybutanoic acid and inorganic polyphosphate

被引：114

作者：

Das, S ^{[1
]}

Lengweiler, UD ^{[1
]}

Seebach, D ^{[1
]}

Reusch, RN ^{[1
]}

机构：

[1] ETH ZURICH,ORGAN CHEM LAB,CH-8092 ZURICH,SWITZERLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 17期

关键词：

bacterial calcium channel; polymer electrolyte complex; planar bilayer; poly(3-hydroxybutyrate);

D O I：

10.1073/pnas.94.17.9075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Traditionally, the structure and properties of natural products have been determined by total synthesis and comparison with authentic samples, We have now applied this procedure to the first nonproteinaceous ion channel, isolated from bacterial plasma membranes, and consisting of a complex of poly(3-hydroxybutyrate) and calcium polyphosphate, To this end, we have now synthesized the 128-mer of hydroxybutanoic acid and prepared a complex with inorganic calcium polyphosphate (average 65-mer), which was incorporated into a planar lipid bilayer of synthetic phospholipids. We herewith present data that demonstrate unambiguously that the completely synthetic complex forms channels that are indistinguishable in their voltage-dependent conductance, in their selectivity for divalent cations, and in their blocking behavior (by La3+) from channels isolated from Escherichia coli, The implications of our finding for prebiotic chemistry, biochemistry, and biology are discussed.

引用

页码：9075 / 9079

页数：5

共 44 条

[31] POLY-3-HYDROXYBUTYRATE/POLYPHOSPHATE COMPLEXES FROM VOLTAGE-ACTIVATED CA2+ CHANNELS IN THE PLASMA-MEMBRANES OF ESCHERICHIA-COLI [J].