Amelogenin Nanoparticles in Suspension: Deviations from Spherical Shape and pH-Dependent Aggregation

被引：29

作者：

Aichmayer, Barbara ^{[1
]}

Wiedemann-Bidlack, Felicitas B. ^{[3
]}

Gilow, Christoph ^{[1
]}

Simmer, James P. ^{[2
]}

Yamakoshi, Yasuo ^{[2
]}

Emmerling, Franziska ^{[4
]}

Margolis, Henry C. ^{[3
]}

Fratzl, Peter ^{[1
]}

机构：

[1] Max Planck Inst Colloids & Interfaces, Dept Biomat, D-14424 Potsdam, Germany

[2] Univ Michigan, Dent Res Lab, Ann Arbor, MI 48109 USA

[3] Forsyth Inst, Dept Biomineralizat, Boston, MA 02115 USA

[4] BAM Fed Inst Mat Res & Testing, D-12489 Berlin, Germany

来源：

BIOMACROMOLECULES | 2010年 / 11卷 / 02期

关键词：

DYNAMIC LIGHT-SCATTERING; ENAMEL MATRIX PROTEINS; SMALL-ANGLE; RECOMBINANT AMELOGENIN; OCTACALCIUM PHOSPHATE; SECRETORY-STAGE; CRYSTAL-GROWTH; NANOSPHERES; ORIENTATION; TEMPERATURE;

D O I：

10.1021/bm900983b

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is well-known that amelogenin self-assembles to form nanoparticles, usually referred to Lis amelogenin nanospheres, despite the fact that not much is known about their actual shape in solution In the Current paper, we combine SAXS and DLS to Study the three-dimensional shape of the recombinant amelogenins rP172 and rM179 Our results show for the first time that amelogenins build oblate nanoparticles in suspension using experimental approaches that do not require the proteins to be in contact with it support material surface. The SAXS studies give evidence for the existence of isolated amelogenin nano-oblates with aspect ratios in the range of 0 45-0 5 at pH values higher than pH 7 2 and show in aggregation of these nano-oblates at lower pH values. The role of the observed oblale shape in the formation of chain-like structures at physiological conditions is discussed its a key factor in the biomineralization of dental enamel

引用

页码：369 / 376

页数：8

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