Characterization and oxidative in vitro cross-linking of an extensin-like protein and a proline-rich protein purified from chickpea cell walls

被引：36

作者：

Otte, O ^{[1
]}

Barz, W ^{[1
]}

机构：

[1] Univ Munster, Inst Biochem & Biotechnol Pflanzen, D-48143 Munster, Germany

来源：

PHYTOCHEMISTRY | 2000年 / 53卷 / 01期

关键词：

Cicer arietinum; Fabaceae; chickpea; cell wall; oxidative cross-linking; oxidative burst; extensin; proline-rich proteins;

D O I：

10.1016/S0031-9422(99)00463-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cell wall proteins from chickpea, known to be rapidly insolubilised by an elicitor-stimulated oxidative burst in-vivo, were purified from suspension cells. N-terminal protein sequencing revealed them as a proline-rich protein and an extensin-like protein. Oxidative cross-linking could be modelled in an in vitro system utilising horseradish peroxidase, H2O2 and the substrate proteins. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1 / 5

页数：5

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