A set of consensus mammalian Mediator subunits identified by multidimensional protein identification technology

被引：259

作者：

Sato, S

Tomomori-Sato, C

Parmely, TJ

Florens, L

Zybailov, B

Swanson, SK

Banks, CAS

Jin, JJ

Cai, Y

Washburn, MP

Conaway, JW

Conaway, RC

机构：

[1] Stowers Inst Med Res, Kansas City, MO 64110 USA

[2] Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS 66160 USA

[3] Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73190 USA

来源：

MOLECULAR CELL | 2004年 / 14卷 / 05期

关键词：

D O I：

10.1016/j.molcel.2004.05.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase 11 (pol 11) transcription by DNA binding transcription factors. In the work described here, we exploit multidimensional protein identification technology (MudPIT) to carry out a proteomic analysis of the subunit composition of the mammalian Mediator complex. By comparing MudPIT data sets obtained from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med25 (MED9), Intersex (MED29), LCMR1 (MEDI 9), AK007855 (MED28), or CRSP70 (MED26) subunits, we identify a set of consensus mammalian Mediator subunits. In addition, we identify as Mediator-associated proteins the CDK8-like cyclin-dependent kinase CDK11 and the TRAP240-like KIAA1025 protein (MED13L), which is mutated in patients with the congenital heart defect transposition of the great arteries (TGA).

引用

页码：685 / 691

页数：7

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