Two subunits of the Drosophila mediator complex act together to control cell affinity

被引:89
作者
Janody, F
Martirosyan, Z
Benlali, A
Treisman, JE
机构
[1] NYU, Sch Med, Skirball Inst Biomol Med, New York, NY 10016 USA
[2] NYU, Sch Med, Dept Cell Biol, New York, NY 10016 USA
来源
DEVELOPMENT | 2003年 / 130卷 / 16期
关键词
TRAP; ARC; transcription; adhesion; compartment; boundary; Drosophila;
D O I
10.1242/dev.00607
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The organizing centers for Drosophila imaginal disc development are created at straight boundaries between compartments; these are maintained by differences in cell affinity controlled by selector genes and intercellular signals. skuld and kohtalo encode homologs of TRAP240 and TRAP230, the two largest subunits of the Drosophila mediator complex; mutations in either gene cause identical phenotypes. We show here that both genes are required to establish normal cell affinity differences at the anterior-posterior and dorsal-ventral compartment boundaries of the wing disc. Mutant cells cross from the anterior to the posterior compartment, and can distort the dorsal-ventral boundary in either the dorsal or ventral direction. The Skuld and Kohtalo proteins physically interact in vivo and have synergistic effects when overexpressed, consistent with a skuld kohtalo double-mutant phenotype that is indistinguishable from either single mutant. We suggest that these two subunits do not participate in all of the activities of the mediator complex, but form a submodule that is required to regulate specific target genes, including those that control cell affinity.
引用
收藏
页码:3691 / 3701
页数:11
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