Role of yeast Rrp1 protein in the dynamics of pre-ribosome maturation

被引：85

作者：

Horsey, EW ^{[1
]}

Jakovljevic, E ^{[1
]}

Miles, TD ^{[1
]}

Harnpicharnchai, P ^{[1
]}

Woolford, JL ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Biol Sci, Mellon Inst 616, Pittsburgh, PA 15213 USA

来源：

RNA | 2004年 / 10卷 / 05期

关键词：

pre-ribosomes; pre-rRNA processing;

D O I：

10.1261/rna.5255804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA(3) to 27SB(S) pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA(3) and 27SB(L) pre-ribosomal RNAs.

引用

页码：813 / 827

页数：15

共 79 条

[1] Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits [J].

Adams, CC ;

Jakovljevic, J ;

Roman, J ;

Harnpicharnchai, P ;

Woolford, JL .

RNA, 2002, 8 (02) :150-165

[2]

Andersen JS, 2002, CURR BIOL, V12, P1, DOI 10.1016/S0960-9822(01)00650-9

[3] YEAST MUTANT DEFECTIVE IN PROCESSING OF 27S R-RNA PRECURSOR [J].

ANDREW, C ;

HOPPER, AK ;

HALL, BD .

MOLECULAR AND GENERAL GENETICS, 1976, 144 (01) :29-37

[4]

Ausubel FM., 1994, Curr. Protoc. Mol. Biol

[5] Identification of a 60S preribosomal particle that is closely linked to nuclear export [J].

Bassler, J ;

Grandi, P ;

Gadal, O ;

Lessmann, T ;

Petfalski, E ;

Tollervey, D ;

Lechner, J ;

Hurt, E .

MOLECULAR CELL, 2001, 8 (03) :517-529

[6] The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis [J].

Basu, U ;

Si, K ;

Warner, JR ;

Maitra, U .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (05) :1453-1462

[7] Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis [J].

Burger, F ;

Daugeron, MC ;

Linder, P .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12) :2315-2323

[8] Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly [J].

Ceci, M ;

Gaviraghi, C ;

Gorrini, C ;

Sala, LA ;

Offenhäuser, N ;

Marchisio, PC ;

Biffo, S .

NATURE, 2003, 426 (6966) :579-584

[9] YEAST NOP2 ENCODES AN ESSENTIAL NUCLEOLAR PROTEIN WITH HOMOLOGY TO A HUMAN PROLIFERATION MARKER [J].

DEBEUS, E ;

BROCKENBROUGH, JS ;

HONG, B ;

ARIS, JP .

JOURNAL OF CELL BIOLOGY, 1994, 127 (06) :1799-1813

[10] Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway [J].

Dechampesme, AM ;

Koroleva, O ;

Leger-Silvestre, I ;

Gas, N ;

Camier, S .

JOURNAL OF CELL BIOLOGY, 1999, 145 (07) :1369-1380

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