Novel Dictyostelium unconventional myosin, MyoM, has a putative RhoGEF domain

被引：16

作者：

Oishi, N

Adachi, H

Sutoh, K

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan

[2] Teikyo Univ, Sch Med, Itabashi Ku, Tokyo 1738605, Japan

[3] Teikyo Univ, Biotechnol Res Ctr, Miyamae Ku, Kawasaki, Kanagawa 2160001, Japan

来源：

FEBS LETTERS | 2000年 / 474卷 / 01期

关键词：

motor protein; myosin; Rho-type GTPase; GDP/GTP exchange factor; MyoM; Dictyostelium discoideum;

D O I：

10.1016/S0014-5793(00)01564-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a novel unconventional myosin gene myoM in Dictyostelium, Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily, Following the motor domain, two calmodulin-binding IQ motifs, a putative coiled-coil region, and a Pro, Ser and Thr-rich domain, lies a combination of dbl homology and pleckstrin homology domains, These are conserved in Rho GDP/GTP exchange factors (RhoGEFs), We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyostelium cells were not affected by the myoM- mutation. Green fluorescent protein-tagged MyoM, however, accumulated at cro,vn-shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis. (C) 2000 Federation of European Biochemical Societies.

引用

页码：16 / 22

页数：7

共 48 条

[1] Dictyostelium IQGAP-related protein specifically involved in the completion of cytokinesis [J].