Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin

被引：9

作者：

Boonserm, P

Angsuthanasombat, C

Lescar, J

机构：

[1] Mahidol Univ, Inst Mol Biol & Genet, Nakhon Pathom 73170, Thailand

[2] Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444904011205

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222(1), with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 Angstrom, and contain one molecule per asymmetric unit. The crystals diffract to similar to2.9 Angstrom using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model.

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页码：1315 / 1318

页数：4

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